Human/Mouse/Rat Endophilin A1/SH3GL2 Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8006T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Endophilin A1/SH3GL2
Ala217-Pro293
Accession # Q99962
Ala217-Pro293
Accession # Q99962
Specificity
Detects human, mouse, and rat Endophilin A1/SH3GL2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Endophilin A1/SH3GL2
Endophilin A1 (also SH3GL2, SH3P4, EEN-B1, CNS-A2, GL2 and SH3D2A) is a 39-42 kDa member of the A subfamily, endophilin (attracted to endocytic protein) family of molecules. It is a neuronally-expressed cytosolic protein that is concentrated in presynaptic areas. Endophilin A1 is a key player in clathrin-associated endocytosis, participating in invagination plus vesicle fission and recycling. It is able to impact membrane curvature through electroststic differences via its N‑terminus, and interact with multiple synaptic region proteins such as dynamin and synaptojanin through an SH3 homology domain at its C-terminus. Although Endophilin A1 is a monomer in the cytosol, it will homodimerize and heterodimerize with endophilin-2 when active at the synapse. Endophilin A1 is also associated with endocytic trafficking of growth factor receptors. In concert with CIN85, Cbl and ataxin-2, Endophilin A1 extend the half-life of EGFR at the plasma membrane. Human Endophilin A1 is 352 amino acids (aa) in length. It contains an aforementioned N-terminal BAR domain (aa 18-249) plus an SH3 domain that binds Pro‑rich aa sequences (aa 290-349). There is one potential alternative start site at Met36. Endophilin A1 is one of three groupA endophilin isoforms, all of which are the product of separate genes. Although the -A2 and -A3 isoforms share approximately 70% aa overall sequence identity with -A1, over aa 217-293, -A2 and -A3 share less than 50% aa sequence identity with Endophilin A1. Over this same aa sequence, human and mouse Endophilin A1 share more that 97% aa sequence identity.
Long Name
SH3-domain GRB2-like 2
Alternate Names
CNSA2, EEN-B1, Endophilin-1, SH3D2A, SH3GL2, SH3P4
Gene Symbol
SH3GL2
UniProt
Additional Endophilin A1/SH3GL2 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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