Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CrkL Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CrkL
Met1-Glu303
Accession # P46109

Specificity

Detects human, mouse, and rat CrkL in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat CrkL Antibody

Detection of Human, Mouse, and Rat CrkL antibody by Western Blot.

Detection of Human, Mouse, and Rat CrkL by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CrkL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5127) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CrkL at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CrkL antibody by Simple WesternTM.

Detection of Human CrkL by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for CrkL at approximately 44 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat CrkL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5127) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human, Mouse and Rat CrkL antibody by Simple WesternTM.

Detection of Human, Mouse and Rat CrkL by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for CrkL at approximately 43 and 44 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat CrkL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5127) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat CrkL Antibody

Application
Recommended Usage

Simple Western

1-20 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 3 reviews rated 3.7 using AF5127 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CrkL

CrkL is a widely-expressed 39 kD adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoetic origin. Through its SH2 and SH3 domains CrkL initiates interactions with numerous downstream proteins such as CBL, Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. It is involved in processes such as adhesion, migration and immune responses. Furthermore, CrkL plays an essential role in Chronic Myelogenous Leukemia and is constitutively phosphorylated by the Bcr-Abl kinase in this disease.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein

Alternate Names

crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Entrez Gene IDs

1399 (Human); 12929 (Mouse); 287942 (Rat)

Gene Symbol

CRKL

UniProt

P46109

Additional CrkL Products

Product Documents for Human/Mouse/Rat CrkL Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat CrkL Antibody

For research use only

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