Detects endogenous human, mouse and rat CDC14A in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PTEN. Reactivity with CDC14B is not known.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Mouse/Rat CDC14 Antibody

Detection of Human and Mouse CDC14 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDC14 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human, Mouse, and Rat CDC14 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, H4-II-E-C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CDC14 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat CDC14 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, H4‑II‑E‑C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDC14

Cell Division Cycle 14 (CDC14) is a dual tyrosine and serine/threonine phosphatase. There are two CDC14 proteins, CDC14A with a molecular weight of 70 kDa, and CDC14B with a molecular weight of 53 kDa. CDC14A is primarily associated with the nucleolus, bound to Cfi1/Net1 during cell cycle interphase. During anaphase and telophase, CDC14 redistributes to the nucleoplasm and cytoplasm, where it plays a role in segregating and separating sister chromatids. Mutations that alter CDC14 function cause uneven distribution of chromosomes in dividing cells.

Long Name

Cell Division Cycle 14

Alternate Names

CDC14A

Entrez Gene IDs

8556 (Human); 229776 (Mouse); 310806 (Rat)

Gene Symbol

CDC14A

UniProt

Q9UNH5

Additional CDC14 Products

Product Documents for Human/Mouse/Rat CDC14 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat CDC14 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat CDC14 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4457

Key Product Details

Species Reactivity

Validated:

Cited:

Applications