Western blot shows lysates of human brain, mouse brain and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Calretinin at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Calretinin in Human Brain.

Calretinin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human/Mouse/ Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Calretinin by Simple Western^TM.

Left: Simple Western lane view shows lysates of human cerebellum, loaded at 1 mg/ml. A specific band was detected for Calretinin at approximately 37 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the human cerebellum.

Applications for Human/Mouse/Rat Calretinin Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Simple Western

2-10 µg/mL
Sample: Human cerebellum

Western Blot

1 µg/mL
Sample: Human brain, mouse brain and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calretinin

Calretinin (also 29 kDa Calbindin and Calbindin 2) is a 30 kDa member of the EF-hand family of Ca²⁺-binding proteins. It serves as both an intracellular calcium sensor and calcium buffer. Calretinin is found in cerebellar granule cells plus cortical bipolar and multipolar GABAergic interneurons.

Human Calretinin is 271 amino acids (aa) in length. It contains six EF-hand domains (aa 16 - 270), each of which show a 12 aa Ca²⁺-binding motif. There are multiple splice variants. One shows a seven aa substitution for aa 181 - 190, accompanied by a 25 aa substitution for the C-terminal Met. Two others show a one and 14 aa substitution for aa 179 - 271, generating a 20 kDa and 22 kDa isoform, respectively. A fourth variant contains a seven aa substitution for aa 185 - 271. Over aa 2 - 177, human and mouse Calretinin show complete aa identity.

Alternate Names

CAB29, CAL2, CALB2

Entrez Gene IDs

794 (Human); 12308 (Mouse); 117059 (Rat)

Gene Symbol

CALB2

UniProt

AAH15484

Additional Calretinin Products

Product Documents for Human/Mouse/Rat Calretinin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat Calretinin Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat Calretinin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5065

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Calretinin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat Calretinin Antibody

Detection of Human/Mouse/Rat Calretinin by Western Blot.