Human/Mouse/Rat alpha-Internexin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4844
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human alpha-Internexin
Val230-Glu450
Accession # Q16352
Val230-Glu450
Accession # Q16352
Specificity
Detects endogenous human, mouse, and rat alpha-Internexin in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat alpha-Internexin Antibody
Detection of Human/Mouse/Rat alpha‑Internexin by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, human, mouse, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat a-Internexin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4844) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha-Internexin at approximately 66 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat alpha-Internexin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line, human, mouse, and rat liver tissue
Sample: SH-SY5Y human neuroblastoma cell line, human, mouse, and rat liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Internexin
Alpha-Internexin ( alpha-INX; also 66 kDa neurofilament protein (NF-66)) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha-INX is one of four Class IV neuronal IF proteins. It both self-assembles, and complexes with NF-L, H and M in select cortical and cerebellar neurons. Human alpha-INX is 499 amino acids (aa) in length. It has a predicted MW of 55 kDa, but runs anomalously at 66 kDa in SDS-PAGE. alpha-INX contains one DNA-binding region (aa 10‑92), a poly-Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha-INX shares more that 96% aa identity with mouse, canine and rat alpha-INX.
Alternate Names
alphaInternexin, INA, NEF5, NF-66, TXBP-1
Gene Symbol
INA
UniProt
Additional alpha-Internexin Products
Product Documents for Human/Mouse/Rat alpha-Internexin Antibody
Product Specific Notices for Human/Mouse/Rat alpha-Internexin Antibody
For research use only
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