Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB93081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for a-Cardiac Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

alpha-Cardiac Actin in Human Heart.

alpha-Cardiac Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat alpha-Cardiac Actin Monoclonal Antibody (Catalog # MAB93081) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human/Mouse/Rat alpha-Cardiac Actin by Simple Western^TM.

Simple Western lane view shows lysates of Human Heart, loaded at 0.2 mg/mL. A specific band was detected for alpha-Cardiac Actin at approximately 49 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat alpha-Cardiac Actin Monoclonal Antibody (Catalog # MAB93081). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat alpha-Cardiac Actin Antibody

Application

Recommended Usage

Immunohistochemistry

1.7-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Simple Western

20 µg/mL
Sample: Human Heart

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Cardiac Actin

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Long Name

Actin, Alpha, Cardiac Muscle 1

Alternate Names

ACTC, ACTC1, ASD5, Cardiac alpha-Actin, CMD1R, CMH11, LVNC4

Entrez Gene IDs

70 (Human); 11464 (Mouse)

Gene Symbol

ACTC1

UniProt

P68032

Additional alpha-Cardiac Actin Products

Product Documents for Human/Mouse/Rat alpha-Cardiac Actin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat alpha-Cardiac Actin Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat alpha-Cardiac Actin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB93081

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all alpha-Cardiac Actin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat alpha-Cardiac Actin Antibody

Detection of Human, Mouse, and Rat alpha-Cardiac Actin by Western Blot.