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Human/Mouse/Rat alpha-Cardiac Actin Antibody
R&D Systems, Inc. a Bio-Techne Brand
Catalog # MAB9308
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Key Product Details
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: |
E.coli-derived recombinant human alpha -Cardiac Actin Asp3-Phe377 Accession # P68032 |
| Applications: | Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2B Clone # 959606 |
| Specificity: |
Detects human alpha -Cardiac Actin in direct ELISAs and Western blots. |
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Western BlotDetection of Human, Mouse, and Rat alpha -Cardiac Actin by Western Blot. Western blot shows lysates of human, mouse, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/ Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Cardiac Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Immunocytochemistryalpha -Cardiac Actin in Human Cardiomyocytes. a-Cardiac Actin was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated with methanol. Specific staining was localized to cytoplasm. |
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Immunohistochemistryalpha -Cardiac Actin in Human Heart. a-Cardiac Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Simple WesternDetection of Human, Mouse, and Rat alpha -Cardiac Actin by Simple WesternTM. Simple Western lane view shows lysates of human, mouse, and rat heart tissue, loaded at 0.2 mg/mL. A specific band was detected for alpha -Cardiac Actin at approximately 50 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat alpha -Cardiac Actin Monoclonal Antibody (Catalog # MAB9308). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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