Western blot shows lysates of human, mouse, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/ Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Cardiac Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

alpha-Cardiac Actin in Human Cardiomyocytes.

a-Cardiac Actin was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated with methanol. Specific staining was localized to cytoplasm.

alpha-Cardiac Actin in Human Heart.

a-Cardiac Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Applications for Human/Mouse/Rat alpha-Cardiac Actin Antibody

Application

Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed human induced pluripotent stem cells (iPSC) differentiated to cardiomyocytes

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Simple Western

2 µg/mL
Sample: Human, mouse, and rat heart tissue

Western Blot

0.1 µg/mL
Sample: Human, mouse, and rat heart tissue

Reviewed Applications

Read 1 review rated 5 using MAB9308 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Cardiac Actin

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Long Name

Actin, Alpha, Cardiac Muscle 1

Alternate Names

ACTC, ACTC1, ASD5, Cardiac alpha-Actin, CMD1R, CMH11, LVNC4

Entrez Gene IDs

70 (Human); 11464 (Mouse)

Gene Symbol

ACTC1

UniProt

P68032

Additional alpha-Cardiac Actin Products

Product Documents for Human/Mouse/Rat alpha-Cardiac Actin Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse/Rat alpha-Cardiac Actin Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat alpha-Cardiac Actin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9308

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat alpha-Cardiac Actin Antibody

Detection of Human, Mouse, and Rat alpha-Cardiac Actin by Western Blot.