Human/Mouse/Rat alpha -Actinin 1 Antibody

Name: Human/Mouse/Rat alpha -Actinin 1 Antibody
Brand: R&D Systems
SKU: MAB8279
Price: 123 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB8279

Citations (1)

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Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human alpha -Actinin 1 Asn272-Thr500 Accession # P12814
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 910301
Specificity:	Detects human alpha -Actinin 1 in ELISA, and human, mouse and rat alpha -Actinin 1 in Western Blot.

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Applications for Human/Mouse/Rat alpha -Actinin 1 Antibody

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	2 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat alpha -Actinin 1 Antibody

	Western Blot Detection of Human, Mouse, and Rat alpha -Actinin 1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat a-Actinin 1 Monoclonal Antibody (Catalog # MAB8279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Mouse alpha -Actinin 1 by Simple Western^TM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.5 mg/mL. A specific band was detected for alpha -Actinin 1 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat alpha -Actinin 1 Monoclonal Antibody (Catalog # MAB8279). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: alpha Actinin 1

alpha -Actinin 1 (ACTN1) is a member of the spectrin superfamily with a predicted molecular weight of approximately 100 kDa. It is 892 amino acids (aa) in length and shares 99% aa identity with mouse and rat ACTN1. Multiple splice forms exist, resulting in 4 distinct isoforms with different tissue expression patterns. ACTN1 binds to F-actin as well as other cytoskeletal and signaling proteins and plays an important role in both cell motility and muscle contraction.

Item	Value
Long Name	Actinin alpha 1
Entrez Gene IDs	87 (Human); 109711 (Mouse); 81634 (Rat)
Alternate Names	ACTN1, Alpha-actinin cytoskeletal isoform, BDPLT15, F-actin cross-linking protein, FLJ40884, FLJ54432, Non-muscle alpha-actinin-1, actinin 1 smooth muscle, actinin, alpha 1, alpha Actinin 1, alpha-Actinin 1, alpha-actinin-1, alphaActinin 1

Citations for Human/Mouse/Rat alpha -Actinin 1 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat alpha -Actinin 1 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Simple Western

A Pyner et al. (2019), Indirect Chronic Effects of an Oleuropein-Rich Olive Leaf Extract on Sucrase-Isomaltase In Vitro and In Vivo Nutrients, 11(7).
PMID: 31266155

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Product Documents for Human/Mouse/Rat alpha -Actinin 1 Antibody

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Human/Mouse/Rat alpha -Actinin 1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse/Rat alpha -Actinin 1 Antibody

Scientific Data Examples for Human/Mouse/Rat alpha -Actinin 1 Antibody

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: alpha Actinin 1

Citations for Human/Mouse/Rat alpha -Actinin 1 Antibody (1)

Product Documents for Human/Mouse/Rat alpha -Actinin 1 Antibody

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