Human/Mouse/Rat Akt2 Antibody

Akt2 in Mouse Spleen.

Akt2 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Akt2 by Simple Western^TM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Akt2 at approximately 60 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Akt2 by Western Blot

The activation of upstream regulators for Rac1 following insulin stimulation in the gastrocnemius muscle of Lepob/ob mice. (A) Insulin was administered by intravenous injection, and the phosphorylation of serine in the C-terminal hydrophobic motif of Akt proteins (serine-474 of Akt2) in gastrocnemius muscle was assessed by immunoblot analysis. Total Akt2 and phosphorylated Akt molecules were detected. (B) Quantification of the phosphorylation level of Akt (p-Akt/Akt2) relative to that in unstimulated wild-type muscle. Data are shown as means ± S.E. (n = 3). ** p < 0.01 (Student’s t-test). (C) Insulin was administered by intravenous injection, and the translocation of FLJ00068 to the plasma membrane in gastrocnemius muscle was assessed by immunoblot analysis. FLJ00068 molecules in cytosolic (C) and plasma membrane (P) fractions were detected. (D) Quantification of the protein expression level of FLJ00068 in the cytosol (FLJ00068/ alpha-tubulin) relative to that in unstimulated wild-type muscle. Data are shown as means ± S.E. (n = 3). (E) Quantification of the protein expression level of FLJ00068 in the plasma membrane (FLJ00068/Na+-K+-ATPase) relative to that in unstimulated wild-type muscle. Data are shown as means ± S.E. (n = 3). ** p < 0.01, *** p < 0.001, (Student’s t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37511290), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Akt2 by Western Blot

The activation of upstream regulators for Rac1 following insulin stimulation in the gastrocnemius muscle of Lepob/ob mice. (A) Insulin was administered by intravenous injection, and the phosphorylation of serine in the C-terminal hydrophobic motif of Akt proteins (serine-474 of Akt2) in gastrocnemius muscle was assessed by immunoblot analysis. Total Akt2 and phosphorylated Akt molecules were detected. (B) Quantification of the phosphorylation level of Akt (p-Akt/Akt2) relative to that in unstimulated wild-type muscle. Data are shown as means ± S.E. (n = 3). ** p < 0.01 (Student’s t-test). (C) Insulin was administered by intravenous injection, and the translocation of FLJ00068 to the plasma membrane in gastrocnemius muscle was assessed by immunoblot analysis. FLJ00068 molecules in cytosolic (C) and plasma membrane (P) fractions were detected. (D) Quantification of the protein expression level of FLJ00068 in the cytosol (FLJ00068/ alpha-tubulin) relative to that in unstimulated wild-type muscle. Data are shown as means ± S.E. (n = 3). (E) Quantification of the protein expression level of FLJ00068 in the plasma membrane (FLJ00068/Na+-K+-ATPase) relative to that in unstimulated wild-type muscle. Data are shown as means ± S.E. (n = 3). ** p < 0.01, *** p < 0.001, (Student’s t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37511290), licensed under a CC-BY license. Not internally tested by R&D Systems.