Western blot shows lysates of A431 human epithelial carcinoma cell line and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3s Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 sigma was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human 14‑3‑3 sigma Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 14-3-3s knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat 14-3-3s Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 14-3-3s at approximately 28 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat 14-3-3 sigma Antibody

Application

Recommended Usage

Knockout Validated

14‑3‑3 sigma is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in 14‑3‑3 sigma knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line and mouse brain tissue

Reviewed Applications

Read 1 review rated 5 using AF4424 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 sigma

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha/ beta, epsilon, eta, gamma, tau/ theta, sigma, and zeta/ delta.

Alternate Names

1433 sigma, Epithelial cell marker protein 1, SFN, YWHAS

Entrez Gene IDs

2810 (Human)

Gene Symbol

SFN

UniProt

P31947

Additional 14-3-3 sigma Products

Product Documents for Human/Mouse/Rat 14-3-3 sigma Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat 14-3-3 sigma Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat 14-3-3 sigma Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4424

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all 14-3-3 sigma Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat 14-3-3 sigma Antibody

Detection of Human/Mouse/ Rat 14‑3‑3 sigma by Western Blot.