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Human/Mouse/Rat 14-3-3 epsilon Antibody

Bio-Techne includes R&D Systems | Catalog # AF4419

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AF4419-SP
AF4419

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human/Mouse/Rat 14-3-3 epsilon Antibody

Immunogen

E. coli-derived recombinant human 14-3-3 epsilon
Met1-Gln255
Accession # P62258

Specificity

Detects human, mouse, and rat 14-3-3 epsilon in Western blots. In Western blots, less than 1% cross-reactivity with other recombinant human 14-3-3 isoforms is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat 14-3-3 epsilon Antibody

Detection of Human/Mouse/ Rat 14-3-3e antibody by Western Blot.

Detection of Human/Mouse/ Rat 14‑3‑3 epsilon by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromo­cytoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14-3-3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat 14-3-3 epsilon Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using AF4419 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 epsilon

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha/ beta, epsilon, eta, gamma, tau/ theta, sigma, and zeta/ delta.

Alternate Names

1433 epsilon, YWHAE

Entrez Gene IDs

7531 (Human); 22627 (Mouse); 29753 (Rat)

Gene Symbol

YWHAE

UniProt

Additional 14-3-3 epsilon Products

Product Documents for Human/Mouse/Rat 14-3-3 epsilon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat 14-3-3 epsilon Antibody

For research use only

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