Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKN1 Monoclonal Antibody (Catalog # MAB6100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse PKN1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and L1.2 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKN1

PKN1 (serine/threonine protein kinase N1), also known as PKN or PRK, is a ubiquitous 120 kDa cytoplasmic kinase of the PKC superfamily. The 942 amino acid (aa) PKN1 contains three HR1 repeats (aa 34‑263), a C2 domain (aa 325‑461), and a protein kinase domain (aa 615‑874). PKN1 can be activated by proteolysis, lipid interaction, or Rho G proteins, and is a downstream effector of RhoA. It promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. In vivo, PKN1 phosphorylates histone H3 at the Thr11 position.

Long Name

Serine/Threonine Protein Kinase N1

Alternate Names

DBK, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1

Entrez Gene IDs

5585 (Human); 320795 (Mouse); 29355 (Rat)

Gene Symbol

PKN1

UniProt

Q16512 (Human)

Additional PKN1 Products

Product Documents for Human/Mouse PKN1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse PKN1 Antibody

For research use only

Citations
Reviews

Human/Mouse PKN1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6100

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PKN1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse PKN1 Antibody

Detection of Human and Mouse PKN1 by Western Blot.