Human/Mouse Oct-3/4 Antibody

Name: Human/Mouse Oct-3/4 Antibody
Brand: R&D Systems
SKU: AF1759
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1759

Citations (36)

(2)

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	E. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu) Accession # Q01860
Applications:	Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Oct-3/4 in direct ELISAs and Western blots.

View Full Technical Details for Human/Mouse Oct-3/4 Antibody

Applications for Human/Mouse Oct-3/4 Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	20 µg/mL	See below
Immunoprecipitation	3 µg/10⁶ cells	NTera‑2 human testicular embryonic carcinoma cell line, see our available Western blot detection antibodies
Chromatin Immunoprecipitation (ChIP)	5 µg/10⁶ cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse Oct-3/4 Antibody

	Western Blot Detection of Human and Mouse Oct‑3/4 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Oct-3/4 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Chromatin Immunoprecipitation (ChIP) Detection of Oct‑3/4-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct-3/4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR.
	Immunocytochemistry Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline phosphatase (ALPL) and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Simple Western Detection of Human Oct‑3/4 by Simple Western^TM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct-3/4 at approximately 50 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Oct-3/4

Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.

Item	Value
References	Scholer, H.R. et al. (1990) Nature 344:435. Scholer, H.R. et al. (1989) EMBO J. 8:2543. Rosner, M.H. et al. (1990) Nature 345:686. Niwa, H. et al. (2000) Nat. Genet. 24:372. Pesce, M. et al. (2001) Stem Cells 19:271.
Long Name	Octamer-binding Protein 3/4
Entrez Gene IDs	5460 (Human); 18999 (Mouse)
Alternate Names	3-Oct, Oct-3/4, Oct4, Otf3g, Otf4, Pou5f1

Citations for Human/Mouse Oct-3/4 Antibody (35)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Oct-3/4 Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP

L Xiong et al. (2022), Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells Elife, 11(0).
PMID: 35621159
Species: Human
Sample Types: Whole Tissue
Applications: IHC

M Simunovic et al. (2022), Inï¿½vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells Cell Stem Cell, 29(6):962-972.e4.
PMID: 35659878
J Lotila et al. (2022), Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient Oncogene, 63(0):102865.
PMID: 35843021
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Ma et al. (2021), Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls Stem Cell Research, 54(0):102435.
PMID: 34182254
Species: Human
Sample Types: Whole Cells
Applications: ICC

P Grönroos et al. (2021), Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions Cells, 10(2).
PMID: 33562615
Species: Human
Sample Types: Whole Cells
Applications: ICC

ME Mor et al. (2021), Neural differentiation medium for human pluripotent stem cells to model physiological glucose levels in human brain Brain research bulletin, 173(0):141-149.
PMID: 34022288
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MS Lee et al. (2021), Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration Science Advances, 7(21).
PMID: 34138734
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Pagliaroli et al. (2021), Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B-related neurodevelopmental disorders Nature Communications, 12(1):6469.
PMID: 34753942
Species: Human
Sample Types: Whole Cells
Applications: ICC

SK Sarvestani et al. (2021), Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 12(1):262.
PMID: 33431859
Species: Human
Sample Types: Whole Cells
Applications: ICC

DZ Hazelbaker et al. (2020), A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells Sci Rep, 10(1):635.
PMID: 31959800
Species: Human
Sample Types: Cell Lysates
Applications: ChIP

NQ Liu et al. (2020), WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation Nature Genetics, 0(0).
PMID: 33318687
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

B Cao et al. (2019), O-GlcNAc transferase activates stem-like cell potential in hepatocarcinoma through O-GlcNAcylation of eukaryotic initiation factor 4E J. Cell. Mol. Med., 0(0).
PMID: 30677218
Species: Human
Sample Types: Tumor Tissue Fluid
Applications: ICC

M Sherman-Sa et al. (2019), SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma Gynecol. Oncol., 0(0).
PMID: 30904337
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

W Zhang et al. (2019), The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation Cell Stem Cell, 24(1):138-152.e8.
PMID: 30609396
Species: Rat
Sample Types: Whole Cells
Applications: ICC

T Hyvärinen et al. (2019), Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures Sci Rep, 9(1):17125.
PMID: 31748598
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

F Vlahova et al. (2019), Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A Sci Rep, 9(1):8126.
PMID: 31148575
Species: Human
Sample Types: Whole Cells
Applications: ICC

X Gao et al. (2018), Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel Stem Cell Res, 31(0):193-196.
PMID: 30099336
Species: Human
Sample Types: Whole Cells
Applications: Differentiation

S Kaur et al. (2018), Small non-coding RNA landscape of extracellular vesicles from human stem cells Sci Rep, 8(1):15503.
PMID: 30341351
Species: Human
Sample Types: Whole Cells
Applications: ICC

A Buskin et al. (2018), Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa Nat Commun, 9(1):4234.
PMID: 30315276
Species: Human
Sample Types: Whole Cells
Applications: ICC

K Alsayegh et al. (2017), Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue Sci Rep, 7(0):45577.
PMID: 28361959
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

(2016), Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9 Stem Cell Res, 17(2):430-432.
PMID: 27879218
Species: Human
Sample Types: Tissue Homogenates
Applications: IHC

(2016), Brain tissue banking for stem cells for our future Sci Rep, 6(0):39394.
PMID: 27991551
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

(2016), Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9 Stem Cell Res, 17(2):441-443.
PMID: 27879221
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Luo et al. (2014), Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells. Sci Rep, 4(0):3779.
PMID: 24445363
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Jumabay M et al. (2014), Pluripotent stem cells derived from mouse and human white mature adipocytes. Stem Cells Transl Med, 3(2):161-71.
PMID: 24396033
Species: Human
Sample Types: Whole Cells
Applications: ICC

Wada et al. (2014), Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Stem Cells Dev, 23(18):2225-36.
PMID: 24380401
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP

Apostolou E et al. (2013), Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming. Cell Stem Cell, 12(6):699-712.
PMID: 23665121
Species: Human
Sample Types: Whole Cells
Applications: ICC

Hayashi et al. (2012), Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. PLoS ONE, 7(9):e45435.
PMID: 23029008
Species: Rabbit
Sample Types: Whole Cells
Applications: ICC

Kubota H et al. (2011), Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties. FASEB J., 25(0):2604-14.
PMID: 21525489
Species: Human
Sample Types: Cell Lysates
Applications: ChIP

Wong RC et al. (2011), A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells. Stem Cells, 29(10):1517-27.
PMID: 21898682
Species: Human
Sample Types: Cell Lysates
Applications: ChIP

Chng Z et al. (2010), SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells. Cell Stem Cell, 6(1):59-70.
PMID: 20074535
Species: Human
Sample Types: Whole Cells
Applications: ICC

Fong H et al. (2008), Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cells, 26(8):1931-8.
PMID: 18388306
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Rajesh</LastName><ForeNam D</Initial et al. (2007), Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 25(2):490-9.
PMID: 17284653
Species: Human
Sample Types: Whole Cells
Applications: ICC

Ware CB et al. (2006), A comparison of NIH-approved human ESC lines. Stem Cells, 24(12):2677-84.
PMID: 16916927
Species: Human
Sample Types: Whole Cells
Applications: ICC

Ware CB et al. (2005), Controlled-rate freezing of human ES cells. BioTechniques, 38(6):879-80, 882-3.
PMID: 16018548

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Customer Reviews for Human/Mouse Oct-3/4 Antibody (2)

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Human/Mouse Oct-3/4 Antibody

Name: Shuba Krishnan

Application: ICC/IF

Sample Tested: iPS2 human induced pluripotent stem cells

Species: Human

Verified Customer | Posted 10/18/2018

Cells: human induced pluripotent stem cellsPrimary antibody: Goat anti-human OCT3/4 (1:200), O/N incubation at 4 degreesSecondary antibody: anti-goat AF568
Human/Mouse Oct-3/4 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Umbilical vein endothelial cells

Species: Human

Verified Customer | Posted 11/23/2017

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Product Documents for Human/Mouse Oct-3/4 Antibody

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Human/Mouse Oct-3/4 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human/Mouse Oct-3/4 Antibody

Scientific Data Examples for Human/Mouse Oct-3/4 Antibody

Western Blot

Chromatin Immunoprecipitation (ChIP)

Immunocytochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Oct-3/4

Citations for Human/Mouse Oct-3/4 Antibody (35)

Customer Reviews for Human/Mouse Oct-3/4 Antibody (2)

Customer Images

Human/Mouse Oct-3/4 Antibody

Human/Mouse Oct-3/4 Antibody

Product Documents for Human/Mouse Oct-3/4 Antibody

Product Datasheets

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