Detects human and mouse Norrin in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Norrin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human/Mouse Norrin Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Norrin (Catalog # 3014-NR) under reducing conditions only
Recombinant Mouse Norrin (Catalog # 3497-NR)

Reviewed Applications

Read 1 review rated 5 using MAB3014 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Norrin

Norrin, also known as Norrie Disease Protein, is a secreted protein that has a C-terminal cysteine-knot motif. Although Norrin is unrelated to Wnt family proteins, it is a Frizzled-4-specific ligand that activates the canonical Wnt pathway through Frizzled-4 and LRP5 (LDL receptor-related protein). Mutations in Norrin are associated with Norrie disease and X-linked familial exudative vitroretinopathy. The amino acid sequence of human Norrin is 100% and 94% identical to rhesus macaque and mouse Norrin, respectively.

Alternate Names

NDP

Entrez Gene IDs

4693 (Human)

Gene Symbol

NDP

UniProt

Q00604

Additional Norrin Products

Product Documents for Human/Mouse Norrin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Norrin Antibody

For research use only

Citations
Reviews

Human/Mouse Norrin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3014

Key Product Details

Species Reactivity

Validated:

Cited:

Applications