Human/Mouse Neuronal Pentraxin R/NPTXR Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4414U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 511527
Product Specifications
Immunogen
Mouse myeloma cell line, NS0-derived human Neuronal Pentraxin R/NPTXR
Ala24-Ala500
Accession # O95502
Ala24-Ala500
Accession # O95502
Specificity
Detects human Neuronal Pentraxin R/NPTXR
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Neuronal Pentraxin R/NPTXR
The Neuronal Pentraxin Receptor (NPTXR; also called NPR) is a 55‑65 kDa, type II transmembrane glycoprotein within the Pentraxin family. NPTXR is co‑expressed and forms heteromultimers with the related, secreted proteins NPTX1 and NPTX2/NARP (1, 2). Human NPTXR is a 500 amino acid (aa) protein that includes a 477 aa extracellular domain (ECD) with one calcium-binding Pentraxin domain. The human NPTXR ECD shares 88% and 89% aa sequence identity with mouse and rat NPTXR, respectively. A 62 kDa soluble form is generated by TACE/ADAM17 proteolytic cleavage C-terminal to the transmembrane segment (3). Soluble forms of 55 kDa and 45 kDa have also been reported (2, 3). NPTXR is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions (1). It is enriched on axonal membranes at excitatory synapses where, with NPTX1, it recruits the GluR4 subunit of AMPA-type glutamate receptors (AMPAR) to promote synaptogenesis (3, 4). Synaptic activity stimulates mGluR1 or mGluR5 receptors, which then activate TACE, causing release of the NPTXR heteromultimer complex from the membrane (3). NPTX1/2 within the complex mediate binding AMPAR, while the soluble (but not transmembrane) form of NPTXR within the complex mediates AMPAR endocytosis and mGluR1/5‑mediated long-term depression (3). NPTXR is reported to be increased in cerebrospinal fluid or serum of Alzheimer’s disease patients as compared to controls (5). Aberrant expression of transmembrane NPTXR has also been reported in small-cell lung cancers (SCLC; 6).
Alternate Names
NPR, NPTXR
Gene Symbol
NPTXR
UniProt
Additional Neuronal Pentraxin R/NPTXR Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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