Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse JunD Monoclonal Antibody (Catalog # MAB5526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for JunD at approximately 40 kDa and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse JunD Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JunD

JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or Fos family members to form the AP1 transcription factor. Unlike c‑Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD is a 347 amino acid (aa), 39 kDa protein (JunD-FL) that has a 303 aa, 34 kDa isoform ( deltaJunD) which begins at aa 44. Over the sequence used as an immunogen, human JunD shares 72% aa identity with mouse and rat JunD.

Long Name

JunD Proto-oncogene

Alternate Names

AP-1

Entrez Gene IDs

3727 (Human); 16478 (Mouse); 24518 (Rat)

Gene Symbol

JUND

UniProt

CAA40010

Additional JunD Products

Product Documents for Human/Mouse JunD Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse JunD Antibody

For research use only

Citations
Reviews

Human/Mouse JunD Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5526

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all JunD Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse JunD Antibody

Detection of Human/Mouse JunD by Western Blot.