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Human/Mouse ING1 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB5758

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MAB5758
MAB5758-SP

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 585915

Product Summary for Human/Mouse ING1 Antibody

Immunogen

E. coli-derived recombinant human ING1
Arg70-Ala184
Accession # Q9UK53

Specificity

Detects human and mouse ING1 in Western blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse ING1 Antibody

Detection of Human and Mouse ING1 antibody by Western Blot.

Detection of Human and Mouse ING1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ING1 Monoclonal Antibody (Catalog # MAB5758) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ING1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse ING1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and HT‑2 mouse T cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ING1

ING1 (inhibitor of growth-1) is a member of the ING family of tumor suppressive and apoptosis-promoting epigenetic regulators of chromatin structure. They interact with and alter the ratio of pro- and anti-apoptotic Bcl-2 family members. The p53 tumor suppressor and its downstream targets can potentiate ING1-induced apoptosis. Human isoforms p33ING1, p24ING1c, p33ING1b and p47ING1a vary in N-terminal sequence, resulting in 279, 210, 235 and 422 aa proteins, respectively. The sequence used as an immunogen is common to all isoforms, and shares 83% and 81% amino acid (aa) identity with mouse and rat ING1, respectively.

Long Name

Inhibitor of Growth 1

Alternate Names

p24ING1c, p33ING1b, p47ING1a

Entrez Gene IDs

3621 (Human); 26356 (Mouse); 306626 (Rat)

Gene Symbol

ING1

UniProt

Additional ING1 Products

Product Documents for Human/Mouse ING1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse ING1 Antibody

For research use only

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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