Human/Mouse GLI-1 Antibody

Detection of Human GLI‑1 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human GLI-1. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GLI-1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GLI‑1 in HeLa Human Cell Line.

GLI-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of GLI-1 by Western Blot

Numb is required for maximal activation of Hh signaling. A Diagram of the Hh signaling cascade. Numb regulates Hh signaling at the level of Ptch1; SmoM2 actives Hh signaling independent of Shh. b, c Hh signaling levels in WT or Numb KO cells were assessed by the transcript levels of the Hh-target genes, Gli1 and Ptch1, via qPCR. d Hh signaling levels in WT cells and Numb KO cells that express Numb-V5 or the blank vector. Hh activity was assessed by Gli1 transcript levels. e, f Western blot analysis and quantification of Gli1 protein levels in WT and Numb KO cells. Prior to harvesting, cells were stimulated with 1 μg/ml Shh in the low serum culture medium for 24 h. g SAG-induced Hh signaling levels in WT cells and Numb KO cells. Prior to harvesting, cells were treated with the indicated doses of SAG in the low serum culture medium for 24 h. h Hh signaling activity in WT cells and Numb KO cells infected with lentiviruses that express SmoM2. All experiments were repeated three to four times with similar results. Data are shown as mean ± SD. Statistics in (b, c, d, f, g, h): Two-way ANOVA with multiple comparisons (Tukey test). ns not significant. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38664376), licensed under a CC-BY license. Not internally tested by R&D Systems.