Detects human FABP4/A‑FABP in direct ELISAs and human and mouse FABP4/A-FABP in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) FABP4, 50% cross-reactivity with recombinant human (rh) FABP3, 15% cross-reactivity with rhFABP3, and no cross-reactivity with rhFABP8 or rhFABP9 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Human/Mouse FABP4/A-FABP Antibody

Detection of Human and Mouse FABP4/A‑FABP by Western Blot.

Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP4/A-FABP Monoclonal Antibody (Catalog # MAB3150) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human/Mouse FABP4/A-FABP ELISA Standard Curve.

Recombinant Human/Mouse FABP4/A-FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A-FABP Monoclonal Antibody (Catalog # MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human/Mouse FABP4/A-FABP Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FABP4/A-FABP DuoSet ELISA Kit (Catalog # DY3150-05) for convenient development of a sandwich ELISA or the Human FABP4 Quantikine ELISA Kit (Catalog # DFBP40) for a complete optimized ELISA.

Western Blot

1 µg/mL
Sample: Human adipose tissue and mouse adipose tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP4/A-FABP

FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.

Long Name

Fatty Acid-Binding Protein 4

Alternate Names

A-FABP, AFABP, aP2

Entrez Gene IDs

2167 (Human); 11770 (Mouse); 79451 (Rat)

Gene Symbol

FABP4

UniProt

P15090

Additional FABP4/A-FABP Products

Product Documents for Human/Mouse FABP4/A-FABP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse FABP4/A-FABP Antibody

For research use only

Citations
Reviews

Human/Mouse FABP4/A-FABP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3150

Key Product Details

Species Reactivity

Validated:

Cited:

Applications