Human/Mouse EBF-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5166
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Zebrafish
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse EBF-3
Ile399-Ser504
Accession # O08791-2
Ile399-Ser504
Accession # O08791-2
Specificity
Detects endogenous human and mouse EBF-3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse EBF-3 Antibody
Detection of Human/Mouse EBF-3 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF-3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse EBF-3 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and CH-1 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line
Sample: Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and CH-1 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EBF-3
EBF-3 (Early B cell Factor 3; also OE2 and COE3) is a 65 kDa (predicted) member of the COE family of transcription factors. It has limited expression, being identified principally in cerebellar Purkinje cells, olfactory neurons, and differentiating mantle layer neurons. Mouse EBF-3 is 596 amino acids (aa) in length it contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 371‑431, and a Pro/Ser-rich transactivation domain (aa 464‑555). EBF-3 either homodimerizes, or heterodimerizes with EBF-2 or -1. There are at least two potential splice variants, one that shows a nine aa deletion between aa 252 - 260, and a second that shows the same deletion coupled to a one aa substitution for aa 521‑557. Over aa 399‑504, mouse EBF-3 shows more than 99% aa identity to rat and human EBF-3.
Long Name
Early B-cell Factor 3
Alternate Names
COE3, EBF3, OE-2
Gene Symbol
EBF3
UniProt
Additional EBF-3 Products
Product Documents for Human/Mouse EBF-3 Antibody
Product Specific Notices for Human/Mouse EBF-3 Antibody
For research use only
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