Western blot shows lysates of human motor cortex tissue, human hypothalamus tissue, and human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Doublecortin/DCX at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Doublecortin/DCX in Mouse Brain.

Doublecortin/DCX was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse Doublecortin/DCX Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)

Western Blot

1 µg/mL
Sample: Human motor cortex tissue, Human hypothalamus tissue, and Human hippocampus tissue

Reviewed Applications

Read 3 reviews rated 4 using AF10025 in the following applications:

Immunocytochemistry/Immunofluorescence (3 Reviews)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Doublecortin/DCX

Doublecortin (DCX) is a 365 aminoacids, 40 kDa microtubule-associated phosphoprotein (MAP) encoded by the DCX gene. DCX is an essential factor in neurogenesis, modulating, stabilizing and stimulating polymerization of microtubules (MTs) to ensure effective migration of neurons of the central and peripheral nervous system during embryonic and postnatal development. Mutations in this protein impairs neuronal migration leading to several pathological conditions.

DCX is very conserved across species. Human and non-human primates DCX proteins are 98-99% identical at the aminoacid level, and 100% identical at the sequences used for immunogen of AF10025; human, mouse and rat DCX aminoacid sequences are 98% identical.

Long Name

Doublecortin

Alternate Names

DBCN, Doublecortex, Doublin, Lis-X, LISX

Entrez Gene IDs

1641 (Human); 13193 (Mouse); 84394 (Rat)

Gene Symbol

DCX

UniProt

O43602

Additional Doublecortin/DCX Products

Product Documents for Human/Mouse Doublecortin/DCX Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Doublecortin/DCX Antibody

For research use only

Citations
Reviews

Human/Mouse Doublecortin/DCX Antibody

R&D Systems, part of Bio-Techne | Catalog # AF10025

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Doublecortin/DCX Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Doublecortin/DCX Antibody

Detection of Human Doublecortin/DCX by Western Blot.