Human/Mouse CDK4 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5254AFP488

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AF5254AFP488-100UG
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG

View all CDK4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CDK4

Specificity

Detects human and mouse CDK4 in direct ELISA.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CDK4

CDK4 (cyclin-dependent kinase 4) is a 34 kDa member of the CMGC Ser/Thr protein kinase family of enzymes. Within the cell, pRb/retinoblastoma protein is normally unphosphorylated and suppresses cell growth at the G1 stage of the cell cycle. When mitosis is required, CDK4 is phosphorylated, forms a complex with cyclin D1, 2 or 3, and phosphorylates pRb/retinoblastoma protein. This inactivates pRb and cells initiate DNA synthesis. Human CDK4 is 303 amino acids (aa) in length. It contains one protein kinase domain (aa 6‑295) and an activating phosphorylation site at Thr172. There are three potential variants; one that shows an alternate start site at Met75, a second that shows a two aa substitution for aa 89‑211, and a third that shows a 23 aa substitution for aa 89‑303. Full-length human and mouse CDK4 are 95% aa identical.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Entrez Gene IDs

1019 (Human); 12567 (Mouse); 94201 (Rat)

Gene Symbol

CDK4

UniProt

P11802

Additional CDK4 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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