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Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 392411

Product Summary for Human/Mouse Cadherin‑13 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cadherin-13
Glu23-Ala692
Accession # P55290

Specificity

Detects human Cadherin-13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) E-Cadherin, rhN-Cadherin, rhP-Cadherin, rhVE-Cadherin, rhCadherin-8, -11, -12 or -17 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse Cadherin‑13 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: NCI‑H460 human large cell lung carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Cadherin‑13 under non-reducing conditions only (Catalog # 3264-CA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using MAB3264 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-13

Cadherin-13, also known as T-cadherin and H-cadherin, is a 105 kDa member of the cadherin family of transmembrane glycoproteins that mediate calcium-dependent intercellular adhesion (1). However, Cadherin-13 is an atypical member, lacking transmembrane and cytosolic domains and containing a GPI moiety that anchors Cadherin-13 to the plasma membrane (1‑2). Human Cadherin-13 is synthesized as a 713 amino acid (aa) precursor that contains a 22 aa signal sequence, a 116 aa propeptide, a 555 aa mature chain, and a second propeptide of 20 aa that is removed in the mature form to reveal the GPI anchor. The mature form contains five cadherin domains and eight potential sites for N-linked glycosylation. Mature human Cadherin-13 shares 96% aa identity with mature mouse Cadherin-13. Cadherin-13 is expressed in various tissues. It is highly expressed in the heart, and in the CNS, Cadherin-13 is expressed in the cerebral cortex, medulla, hippocampus, amygdala, thalamus, and substantia nigra (2). There are higher levels of Cadherin-13 in the adult brain than in developing brain (2). Cadherin-13 is also expressed in skin in the basal layer of the epidermis, lung, liver, kidney, and blood vessels (2). The structural characteristics of Cadherin-13 predict that it is unlikely to function as a true adhesion molecule in vivo (2). It is suggested that it may act rather as a signaling receptor participating in recognition of the environment and regulation of cell motility, proliferation, and phenotype (2). Cellular expression levels of Cadherin-13 in various tissues often correlate, negatively or positively, with the proliferative potential of the cells (2). Cadherin-13 may also act as a suppressor of tumor cell growth (2). This potential role for Cadherin-13 was emphasized by localization of Cadherin-13 gene to chromosome 16q24, a region exhibiting loss of heterozygosity in many solid tumors (2). Allelic loss of chromosome bands 16q24.1-q24.2 and reduced expression of Cadherin-13, as well as hypermethylation of the remaining allele have been detected in a considerable number of human cancers (2).

References

  1. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
  2. Philippova, M. et al. (2009) Cell. Signal. 21:1035.

Alternate Names

Cadherin13, CDH13, H-Cadherin, T-Cadherin

Entrez Gene IDs

1012 (Human); 12554 (Mouse); 192248 (Rat)

Gene Symbol

CDH13

UniProt

Additional Cadherin-13 Products

Product Documents for Human/Mouse Cadherin‑13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Cadherin‑13 Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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