Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Apolipoprotein A-IV/ApoA4 Antibodies »

Product Specifications

Immunogen

E. coli-dervied recombinant human Apolipoprotein A-IV/ApoA
Glu21-Ser396
Accession # P06727

Specificity

Detects human Apolipoprotein A-IV/ApoA in direct ELISAs. Detects human and mouse in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody

Detection of Human Apolipoprotein A-IV/ApoA4 antibody by Western Blot.

Detection of Human Apolipoprotein A-IV/ApoA4 by Western Blot.

Western blot shows human plasma and human serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Apolipoprotein A-IV/ApoA4 antibody by Western Blot.

Detection of Mouse Apolipoprotein A-IV/ApoA4 by Western Blot.

Western blot shows mouse plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Apolipoprotein A-IV/ApoA4 antibody in Human Small Intestine by Immunohistochemistry (IHC-P).

Apolipoprotein A-IV/ApoA4 in Human Small Intestine.

Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Applications for Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small intestine and human descending colon

Simple Western

2 µg/mL
Sample: Human Plasma

Western Blot

0.2-2 µg/mL
Sample: Human plasma, human serum, and mouse plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-IV/ApoA4

Apolipoprotein A4 (ApoA4) is a 45 kDa glycoprotein of the lipid transport system.  Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption.  Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis.  ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well.  Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 (aa 21-396) shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

Alternate Names

Apolipoprotein AIV

Entrez Gene IDs

337 (Human); 11808 (Mouse); 25080 (Rat)

Gene Symbol

APOA4

UniProt

P06727

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody

For research use only

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