Skip to main content

Human/Mouse Acetyl-CoA Carboxylase  alpha/ACACA Antibody

Bio-Techne includes R&D Systems | Catalog # MAB6898

Catalog #
Availability
Size / Price
Qty
Loading...
MAB6898-SP
MAB6898

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 738421

Product Summary for Human/Mouse Acetyl-CoA Carboxylase  alpha/ACACA Antibody

Immunogen

E. coli-derived recombinant human Acetyl-CoA Carboxylase alpha/ACACA
Pro1185-Phe1352
Accession # Q13085

Specificity

Detects human Acetyl-CoA Carboxylase alpha/ACACA in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse Acetyl-CoA Carboxylase  alpha/ACACA Antibody

Detection of Human and Mouse Acetyl-CoA Carboxylase a/ACACA antibody by Western Blot.

Detection of Human and Mouse Acetyl-CoA Carboxylase alpha/ACACA by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, HCT-116 human colorectal carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Acetyl-CoA Carboxylase a/ACACA Monoclonal Antibody (Catalog # MAB6898) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Acetyl-CoA Carboxylase a/ACACA at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Acetyl-CoA Carboxylase  alpha/ACACA Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, HCT‑116 human colorectal carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using MAB6898 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Acetyl-CoA Carboxylase alpha/ACACA

ACAC-A (Acetyl-CoA carboxylase alpha/1; also ACC-1 and biotin carboxylase) is a 260-265 kDa cytoplasmic, phosphorylated biotinyl-enzyme. It is widely expressed, and found to be concentrated in hepatocytes, adipocytes and lactating mammary epithelium. It is one of two gene products (ACAC-B/beta being the other) that catalyze the formation of malonyl-CoA from acetyl-CoA. The formation of malonyl-CoA by ACAC-A is a rate-limiting step in fatty acid synthesis; malonyl-CoA formed by ACAC-B acts as a regulator of CPT-1 during fatty acidoxidation. Human ACAC-A is 2346 amino acids (aa ) in length. It contains an N-terminal acetylated Met, one ATP-Grasp domain (aa 275-466) with an embedded biotin carboxylation domain (aa 117-618), a biotinyl-binding region (aa 752-818), and a carboxyltransferase domain (aa 1698-2194). There are at least 17 utilized phosphorylation sites, and two acetylated Lys. ACAC-A exists as either a dimer or higher-order oligomer. Multiple splice variants exist. One possesses an alternative start site at Met79, a second utilizes an alternative start site 37 aa upstream of the standard site, and a third (called PIII) shows a 17 aa substitution for aa 1-75. Over aa 1185-1352, human ACAC-A shares 95% aa identity with mouse ACAC-A, and 97% aa identity with both ovine and bovine ACAC-A.

Long Name

Acetyl-Coenzyme A Carboxylase alpha

Alternate Names

ACACA, ACC1, ACCA, AcetylCoA Carboxylase alpha

Entrez Gene IDs

31 (Human); 107476 (Mouse); 60581 (Rat)

Gene Symbol

ACACA

UniProt

Additional Acetyl-CoA Carboxylase alpha/ACACA Products

Product Documents for Human/Mouse Acetyl-CoA Carboxylase  alpha/ACACA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Acetyl-CoA Carboxylase  alpha/ACACA Antibody

For research use only

Loading...
Loading...
Loading...
Loading...