Detects both pro and active forms of human MMP-8 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP-1, -2, -3, -7, -9, -10, -12, or -13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human MMP-8 Antibody

MMP-8 in Human Breast Cancer Tissue.

MMP-8 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 8 µg/mL Mouse Anti-Human MMP-8 Monoclonal Antibody (Catalog # MAB9081) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in terminal ductules (round) and intralobular duct (elongated) composing glandular lobules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Human MMP-8 ELISA Standard Curve.

Recombinant Human MMP-8 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP-8 Monoclonal Antibody (MAB908) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human MMP-8 Monoclonal Antibody (Catalog # MAB9081) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Detection of MMP-8 in Jurkat cells by Flow Cytometry

Jurkat cells were stained with Mouse Anti-Human MMP-8 Monoclonal Antibody (Catalog # MAB9081, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human MMP-8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MMP‑8 Monoclonal Antibody (Catalog # MAB908).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total MMP-8 DuoSet ELISA Kit (Catalog # DY908) for convenient development of a sandwich ELISA or the Human Total MMP-8 Quantikine ELISA Kit (Catalog # DMP800) for a complete optimized ELISA.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-8

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta-casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis,
MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated.

Long Name

Matrix Metalloproteinase 8

Alternate Names

Collagenase 2, MMP8

Entrez Gene IDs

4317 (Human); 17394 (Mouse); 63849 (Rat)

Gene Symbol

MMP8

UniProt

AAZ38714

Additional MMP-8 Products

Product Documents for Human MMP-8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MMP-8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry