Detects human MMP‑15/MT2‑MMP in direct ELISAs and Western blots. Does not detect the catalytic domain of E. coli-expressed recombinant human MMP-15 (aa 132‑304).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human MMP-15/MT2-MMP Antibody

Immersion fixed paraffin-embedded sections of human placenta

MMP-15/MT2-MMP was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human MMP-15/MT2-MMP Monoclonal Antibody (Catalog # mab9161) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MMP-15/MT2-MMP Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

0.25-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: SW480 human colorectal adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample:

Recombinant Human MMP-15 Catalytic Domain

Reviewed Applications

Read 2 reviews rated 3.5 using MAB9161 in the following applications:

ELISA (1 Review)
Microarray (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-15/MT2-MMP

MMP-15, also known as membrane-type 2 (MT2) MMP, is a cell surface endopeptidase that plays a role in tumor invasion and metastasis.

Long Name

Matrix Metalloproteinase 15/Membrane Type 2 MMP

Alternate Names

MMP15, MT2-MMP

Entrez Gene IDs

4324 (Human)

Gene Symbol

MMP15

Additional MMP-15/MT2-MMP Products

Product Documents for Human MMP-15/MT2-MMP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MMP-15/MT2-MMP Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry