U2OS human osteosarcoma cell line was untreated (-) or treated (+) with proteasome inhibitor LLnV for 1 hour. MDM2 was immunoprecipitated from 500 µg of cell lysate following incubation with 2-3 µg Mouse Anti-Human MDM2 Monoclonal Antibody (Catalog # MAB1244) for 1 hour on ice. MDM2-antibody complexes were absorbed using goat anti-mouse agarose (Sigma). Immunoprecipitated MDM2 was detected by Western blot using 1 µg/mL Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244). View our recommended buffer recipes for immunoprecipitation.

Applications for Human MDM2/HDM2 Antibody

Application

Recommended Usage

Immunoprecipitation

2.5 µg/500 µg cell lysate
Sample: U2OS human osteosarcoma cell line treated with proteasome inhibitor LLnV, see our available Western blot detection antibodies

Reviewed Applications

Read 3 reviews rated 5 using MAB1244 in the following applications:

Western Blot (3 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDM2/HDM2

MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally upregulates the expression of its own inhibitor, MDM2.

Long Name

Double Minute 2 Protein

Alternate Names

HDM2

Entrez Gene IDs

4193 (Human); 17246 (Mouse)

Gene Symbol

MDM2

UniProt

Q00987

Additional MDM2/HDM2 Products

Product Documents for Human MDM2/HDM2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MDM2/HDM2 Antibody

For research use only

Citations
Reviews

Human MDM2/HDM2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1244

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all MDM2/HDM2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MDM2/HDM2 Antibody

Immunoprecipitation of Human MDM2.