Skip to main content

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 602901

Product Specifications

Immunogen

E. coli-derived recombinant human Mcl-1
Met231-Arg350
Accession # Q07820

Specificity

Detects human Mcl-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Mcl-1 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Mcl-1

Mcl-1 (myeloid cell leukemia-1) is a member of the Bcl-2 family of proteins. Alternative splicing creates two distinct isoforms. The 40 kDa, 350 amino acid (aa) isoform 1, also known as Mcl-1L (long), enhances cell survival by inhibiting apoptosis, while the ~35 kDa, 271 aa isoform 2, also known as Mcl-1S (short; with divergence in the last 41 aa) promotes apoptosis. The elimination of Mcl-1L is a required step for DNA damage-induced apoptosis. Mcl-1 can be modified by phosphorylation on S121 and T163 by JNK, which triggers apoptosis, or polyubiquitination, which enhances degradation. Within aa 231-350 (unique to Mcl-1L), human Mcl-1 shares 93% and 96% aa identity with mouse and rat Mcl-1, respectively.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Entrez Gene IDs

4170 (Human); 17210 (Mouse)

Gene Symbol

MCL1

UniProt

Additional Mcl-1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Mcl-1 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Loading...