Human MBL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2307
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Bacteria - Moraxella catarrhalis, Schistosoma mansoni
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Western Blot, Neutralization, Flow Cytometry, Bioassay, ELISA Capture, ELISA Detection, ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MBL
Glu21-Ile248
Accession # AAH96182
Glu21-Ile248
Accession # AAH96182
Specificity
Detects human MBL in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) MBL and rmMBL‑2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MBL Antibody
Human MBL ELISA Standard Curve
Human MBL was serially diluted and captured by Goat Anti-Human MBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2307) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2307) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Human MBL ELISA Standard Curve
Human BMP-9 was serially diluted and captured by Goat Anti-Human MBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2307) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2307) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human MBL Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human MBL (Catalog # 2307-MB)
Sample: Recombinant Human MBL (Catalog # 2307-MB)
Human MBL Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
Use in combination with these reagents:
- Detection Reagent: Human MBL Biotinylated Antibody (Catalog # BAF2307)
- Standard: Recombinant Human MBL Protein (Catalog # 2307-MB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using AF2307 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MBL
MBL belongs to the collectin family of innate immune defense proteins, which bind to an array of carbohydrate patterns on pathogen surfaces. MBL homotrimerizes to form a structural unit that further associates into oligomeric structures. Circulating MBL is complexed with three MBL-associated serine proteases (MASPs). Human MBL shares approximately 60% amino acid sequence identity with mouse MBL1 or MBL2.
References
Long Name
Mannose Binding Lectin
Alternate Names
COLEC1, Collectin-1, MBL2, MBL2D, MBP1
Entrez Gene IDs
4153 (Human)
Gene Symbol
MBL2
UniProt
Additional MBL Products
Product Documents for Human MBL Antibody
Product Specific Notices for Human MBL Antibody
For research use only
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