Detects human MBL in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) MBL and rmMBL‑2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human MBL Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human MBL (Catalog # 2307-MB)

Human MBL Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human MBL Biotinylated Antibody (Catalog # BAF2307)
Standard: Recombinant Human MBL Protein (Catalog # 2307-MB)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using AF2307 in the following applications:

ELISA (1 Review)
Simple Western (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBL

MBL belongs to the collectin family of innate immune defense proteins, which bind to an array of carbohydrate patterns on pathogen surfaces. MBL homotrimerizes to form a structural unit that further associates into oligomeric structures. Circulating MBL is complexed with three MBL-associated serine proteases (MASPs). Human MBL shares approximately 60% amino acid sequence identity with mouse MBL1 or MBL2.

References

Long Name

Mannose Binding Lectin

Alternate Names

COLEC1, Collectin-1, MBL2, MBL2D, MBP1

Entrez Gene IDs

4153 (Human)

Gene Symbol

MBL2

UniProt

AAH96182

Additional MBL Products

Product Documents for Human MBL Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MBL Antibody

For research use only

Citations
Reviews

Human MBL Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2307

Key Product Details

Species Reactivity

Validated:

Cited:

Applications