Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP-1 beta subunit Monoclonal Antibody (Catalog # MAB2368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for LRP-1 beta subunit at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

LRP-1 beta subunit in Human Brain (Cortex).

LRP-1 beta subunit was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human LRP-1 beta subunit Monoclonal Antibody (Catalog # MAB2368) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human LRP‑1 beta subunit Antibody

Application

Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

2 µg/mL
Sample: U-87 MG human glioblastoma/astrocytoma cell line and U-118-MG human glioblastoma/astrocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-1B

LRP-1 (low-density lipoprotein receptor-related protein 1) is a large type I transmembrane protein belonging to the LDL receptor superfamily. The extracellular domain of LRP-1 is organized in four clusters of ligand-binding repeats that recognizes at least 30 different ligands. The amino acid sequence of human and mouse LRP-1 cluster II share 100% identity. LRP-1 is an endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. It plays a crucial role in early embryonic development.

Long Name

LDL Receptor-related Protein 1B

Alternate Names

LRP-DIT, LRP1B

Entrez Gene IDs

53353 (Human); 94217 (Mouse)

Gene Symbol

LRP1B

UniProt

Q07954 (Human)

Additional LRP-1B Products

Product Documents for Human LRP‑1 beta subunit Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LRP‑1 beta subunit Antibody

For research use only

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