Human Lipocalin-2/NGAL Antibody

Name: Human Lipocalin-2/NGAL Antibody
Brand: R&D Systems
SKU: MAB1757
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1757

Citations (11)

(2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Lipocalin-2/NGAL Gln21-Gly198 Accession # P80188
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 220310
Specificity:	Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Lipocalin-1 or rmLipocalin-2. This antibody also recognizes human Lipocalin‑2/MMP‑9 complexes in Western blots under non-reducing conditions.

View Full Technical Details for Human Lipocalin-2/NGAL Antibody

Applications for Human Lipocalin-2/NGAL Antibody

	Recommended Concentration	Sample
Western Blot	0.2-1 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Lipocalin-2/NGAL Antibody

	Western Blot Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry Lipocalin‑2/NGAL in Human Pancreas. Lipocalin-2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Western Blot Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and human bone marrow tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Lipocalin-2/NGAL

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3-5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K_d = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_d values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

Item	Value
References	Flower, D.R. et al. (1994) FEBS Lett. 354:7. Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272. Devireddy, L.R. et al. (2001) Science 293:829. Yang, M.B. et al. (2002) Mol. Cell. 10:1045. Goetz, D.H. et al. (2002) Mol. Cell 10:1033. Flo, T.H. et al. (2004) Nature 432:917. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
Long Name	Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs	3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names	24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, Lipocalin-2, Lipocalin2, MSFI, NGAL, NGALlipocalin-2, Oncogene 24p3, Siderocalin, Uterocalin, lipocalin 2, lipocalin 2 (oncogene 24p3), migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, p25

Citations for Human Lipocalin-2/NGAL Antibody (11)

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Citations are publications that use Bio-Techne products. Selected citations for Human Lipocalin-2/NGAL Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

F Lunardi et al. (2023), Molecular Profiling of Tissue Samples with Chronic Rejection from Patients with Chronic Lung Allograft Dysfunction: A Pilot Study in Cystic Fibrosis Patients Biomolecules, 13(1).
PMID: 36671482
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

F Yao et al. (2021), A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 12(1):7333.
PMID: 34921145
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

I Zhang et al. (2020), Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures Cells, 9(11).
PMID: 33171886
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S An et al. (2019), Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies Ocul Surf, 0(0).
PMID: 30965123
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Bi et al. (2013), Reactive astrocytes secrete lcn2 to promote neuron death. Proc Natl Acad Sci U S A, 110(10):4069-74.
PMID: 23431168
Species: Human
Sample Types: Whole Cells
Applications: Western Blot

Mir SU et al. (2012), Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1. J. Biol. Chem., 287(18):14494-501.
PMID: 22418433
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Mannelqvist M et al. (2012), Lipocalin 2 expression is associated with aggressive features of endometrial cancer. BMC Cancer, 12(0):169.
PMID: 22559235
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Smith ER et al. (2008), Urinary biomarkers predict brain tumor presence and response to therapy. Clin. Cancer Res., 14(8):2378-86.
PMID: 18413828
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Guttman-Yassky E et al. (2008), Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis. J. Immunol., 181(10):7420-7.
PMID: 18981165
Species: Human
Sample Types: Urine
Applications: Western Blot

Pitashny M et al. (2007), Urinary lipocalin-2 is associated with renal disease activity in human lupus nephritis. Arthritis Rheum., 56(6):1894-903.
PMID: 17530720
Species: Human
Sample Types: Synovial Fluid
Applications: Western Blot

Gupta K et al. (2007), Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9. Arthritis Rheum., 56(10):3326-35.
PMID: 17907186

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Customer Reviews for Human Lipocalin-2/NGAL Antibody (2)

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Human Lipocalin-2/NGAL Antibody

Name: Anonymous

Application: IHC

Sample Tested: Lung tissue

Species: Human

Verified Customer | Posted 10/12/2021

Formalin-fixed lung sections
Human Lipocalin‑2/NGAL Antibody

Name: Anonymous

Application: WB

Sample Tested: A549 human lung carcinoma cell line

Species: Human

Verified Customer | Posted 02/24/2016

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Human Lipocalin-2/NGAL Antibody

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Applications for Human Lipocalin-2/NGAL Antibody

Scientific Data Examples for Human Lipocalin-2/NGAL Antibody

Western Blot

Immunohistochemistry

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Lipocalin-2/NGAL

Citations for Human Lipocalin-2/NGAL Antibody (11)

Customer Reviews for Human Lipocalin-2/NGAL Antibody (2)

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Human Lipocalin-2/NGAL Antibody

Human Lipocalin‑2/NGAL Antibody

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