Human LIN-28B Antibody

Catalog # AF7726 | R&D Systems, Inc. a Bio-Techne Brand
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AF7726
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Summary for Human LIN-28B Antibody

Immunogen

E. coli-derived recombinant human LIN-28B
Val83-Thr250
Accession # Q6ZN17

Specificity

Detects human LIN-28B in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human LIN-28A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LIN-28B Antibody

Detection of Human LIN-28B antibody by Western Blot.

Detection of Human LIN-28B by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LIN-28B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7726) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LIN-28B at approximately 22 and 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LIN-28B antibody in Human Liver by Immunohistochemistry (IHC-P).

LIN-28B in Human Liver.

LIN-28B was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human LIN-28B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7726) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human LIN-28B Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIN-28B

Lin-28B (Lineage protein 28 homolog B) is a 35-37 kDa member of the Lin28 family of proteins. Although there is an accompanying Lin-28/28A molecule, Lin-28 and Lin-28B are not alternative splice forms but represent products of separate genes. Lin-28B is found in both the cytoplasm and nucleus of fetal hematopoietic stem and progenitor cells, embryonic liver and multiple tumor types. It is considered a regulator of miRNA activity. Active miRNAs arise from transcribed RNAs that are first cleaved by nuclear RNase III, next cleaved by cytoplasmic Dicer, and finally associate with Argonaute to generate a RISC RNA silencing complex. Lin-28A participates in the cytoplasmic uridylation of miRNAs on their 3’-end, blocking Dicer processing and initiating RNA degradation. Lin-28B, by contrast, acts in the nucleus and blocks the initial RNase III cleavage step. Human Lin-28B is 250 amino acids (aa) in length (SwissProt # Q6ZN17). It contains one CSD/cold shock domain (aa 29-102) plus two CCHC-type Zn-finger RNA bindingdomains (aa 127-144 and 149-166). Phosphorylation is known to occur on Ser105. There is one reported alternate start site at Met71, and there is a potential 11 aa substitution for aa 1-4. Two isoforms of LIN28B with apparent MWs of 22 kDa and 37 kDa have been reported. Over aa 83-250, human Lin-28B shares 84% aa sequence identity with mouse Lin-28B.

Long Name

LIN-28 Homolog B

Alternate Names

CSDD2, Lin-28.2, LIN28B

Entrez Gene IDs

389421 (Human); 380669 (Mouse); 689054 (Rat)

Gene Symbol

LIN28B

UniProt

Q6ZN17

Product Documents for Human LIN-28B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LIN-28B Antibody

For research use only

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