Human LIF Antibody

Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody.

Recombinant Human LIF (Catalog # 7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by 0.5 ng/mL Recom-binant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Polyclonal Antibody (Catalog # AB-250-NA). The ND₅₀ is typically 0.1-0.4 µg/mL.

Detection of LIF by Western Blot

LIF neutralizing antibody inhibits the migration and invasion capability and the Stat3 phosphorylation induced by CAA-CM on breast cancer cells. (A and B) MDA-MD-231 cells cultured in Adi-CM or CAA-CM or control DMEM were treated with LIF neutralizing antibody/IgG. The cell migration (A) and invasion (B) situation were monitored under a phase contrast microscope. The corresponding quantization chart is shown on the right. (C) MDA-MB-231 cells were treated with different culture media for 15 min, and Stat3 phosphorylation was analyzed by western blot. (D) MDA-MD-231 cells cultured in DMEM or Adi-CM or CAA-CM were treated with LIF neutralizing antibody or IgG for 15 min, and Stat3 phosphorylation was analyzed by western blot. Typical microscopic fields and blots are shown and quantitative data are presented as mean ± SD from at least three independent experiments. #p < 0.05, ##p < 0.01, ###p < 0.001, CAA-CM VS. Mock; *p < 0.05, **p < 0.01, ***p < 0.001, CAA-CM + alpha-LIF VS. CAA-CM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35280694), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of LIF by Immunohistochemistry

Knockdown of endogenous Stat3 with siRNA inhibits the migration and invasion of breast cancer cells induced by CAA-CM and rhLIF. (A-D) MDA-MB-231 cells transfected with siRNA were treated with rhLIF (A and B) or CAA-CM (C and D) to observe cell migration and invasion. (E) The expression of LIF and Stat3 phosphorylation in normal breast tissue and breast cancer tissue was analyzed by IHC, scale bar: 100 μm. (F) The correlation between the relative expression of LIF and Stat3 phosphorylation in human breast cancer sections. Typical microscopic fields and blots are shown and quantitative data are presented as mean ± SD from at least three independent experiments. #p < 0.05, ##p < 0.01, ###p < 0.001, NS + rhLIF or CAA-CM VS. NS; *p < 0.05, **p < 0.01, ***p < 0.001, siRNA+rhLIF or CAA-CM VS. NS + rhLIF or CAA-CM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35280694), licensed under a CC-BY license. Not internally tested by R&D Systems.