This antibody functions as an ELISA detection antibody when paired with human anti-Leptin R Recombinant Monoclonal Antibody (Catalog # MAB8671R) . This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Leptin R DuoSet ELISA (Catalog # DY389) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript, lacking a transmembrane domain and potentially encoding a soluble form of the receptor, has also been described. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Human OB-R long form encodes a 1165 amino acid (aa) precursor protein with a 22 aa signal peptide, an 819 aa extracellular domain, a 21 aa transmembrane domain and a 303 aa cytoplasmic domain. The extracellular domain of OB-R contain two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant soluble OB-R has been shown to bind Leptin with high affinity and is a potent Leptin antagonist.

References

Tartaglia, L.A. et al. (1995) Cell 83:1263.
Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.

Long Name

Leptin Receptor

Alternate Names

B219, CD295, LEPR, LeptinR, OB R

Entrez Gene IDs

3953 (Human); 16847 (Mouse)

Gene Symbol

LEPR

UniProt

P48357

Additional Leptin R Products

Product Documents for Human Leptin R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Leptin R Antibody

For research use only

Citations
Reviews

Human Leptin R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB86713

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Leptin R Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for Human Leptin R Antibody

ELISA