Human Lck Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3704
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Lck
Met1-Val66
Accession # P06239
Met1-Val66
Accession # P06239
Specificity
Detects human Lck in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Lck Antibody
Detection of Human Lck by Western Blot.
Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human Lck Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3704) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Lck at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lck in Human Thymus.
Lck was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human Lck Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3704) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human Lck by Simple Western
TIM-3 Association with Intracellular Kinases in CD8+/MART-1+ T cells.TIM-3 co-immunoprecipitation analysis of unactivated and 15 min. stimulation with anti-CD3/CD28 beads (activated). Equivalent amounts of protein (~2mg) were co-immunoprecipitated with pAb anti-TIM-3 antibody and western blot was performed using capillary electrophoresis. Cleared lysate served as a loading control for individual antibody reactivity. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0140694), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Lck Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thymus
Sample: Immersion fixed paraffin-embedded sections of human thymus
Simple Western
5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Western Blot
0.5 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lck
Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). Within aa 2-66, human Lck shares 89% aa sequence identity with mouse and rat Lck. A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene.
Long Name
Lymphocyte Protein Tyrosine Kinase
Alternate Names
Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16
Gene Symbol
LCK
UniProt
Additional Lck Products
Product Documents for Human Lck Antibody
Product Specific Notices for Human Lck Antibody
For research use only
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