Human Laminin alpha3/Laminin-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11784
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # P3H9-2R
Product Specifications
Immunogen
Human keratinocytes
Accession # Q16787
Accession # Q16787
Specificity
Detects recombinant human Laminin alpha3 protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for Human Laminin alpha3/Laminin-5 Antibody
Detection of Laminin5 in frozen human skin.
Laminin alpha3/Laminin-5 was detected in perfusion fixed frozen sections of human skin using Mouse Anti-Human Laminin alpha3/Laminin-5 Monoclonal Antibody (Catalog # MAB11784) at 1.5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane in frozen human Skin. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Laminin alpha3/Laminin-5 in A431 cells.
Laminin alpha3/Laminin-5 was detected in immersion fixed A431 human epithelial carcinoma cell line (Positive) and absent in Daudi human Burkitt's lymphoma cell line (Negative) using Mouse Anti-Human Laminin alpha3/Laminin-5 Monoclonal Antibody (Catalog # MAB11784) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Laminin alpha3/Laminin-5 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Immunohistochemistry
1-25 µg/mL
Sample: Perfusion frozen fixed sections of human Skin.
Sample: Perfusion frozen fixed sections of human Skin.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin alpha 3/Laminin-5
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival. Laminin alpha3/Laminin-5, also known as epiligrin, includes alpha3, beta3, and gamma2 subunits. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and contributes to initiation and maintenance of epithelial cell anchorage to the underlying connective tissue. Within aa 21‑1713 of the alpha3 subunit, human and mouse share 77% amino acid sequence identity.
Alternate Names
Epiligrin, LAMA3, Laminin-5, Laminin5
Entrez Gene IDs
3909 (Human)
Gene Symbol
LAMA3
UniProt
Additional Laminin alpha 3/Laminin-5 Products
Product Documents for Human Laminin alpha3/Laminin-5 Antibody
Product Specific Notices for Human Laminin alpha3/Laminin-5 Antibody
For research use only
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