Detects human Lactase-like Protein/LCTL in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Lactase-like Protein/LCTL is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Lactase-like Protein/LCTL Antibody

Detection of Human Lactase-like Protein/LCTL by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Lactase-like Protein/LCTL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5984) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lactase-like Protein/LCTL at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Lactase-like Protein/LCTL Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Lactase-like Protein/LCTL, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lactase-like Protein/LCTL

LCTL (also Lactase-like Protein and Klotho/lactase-phlorizin hydrolase-related protein/KLPH) is a 62 kDa (predicted) member of the glycosyl hydrolase 1 family of proteins. It is expressed in skin and kidney, and may participate in endocrine FGF19 subfamily signaling pathways. Mature human LCTL is a 546 amino acid (aa) type I transmembrane protein that appears to be present in the ER. It contains an apparently nonfunctional glycosidase domain (aa 33‑503), plus a very short five aa cytoplasmic segment that may function as an ER-retention signal. There is one potential alternative start site at Met174. Over aa 23‑541, human LCTL shares 86% aa identity with mouse LCTL.

Alternate Names

KLG, KLPH, Lactase like Protein, LCTL

Entrez Gene IDs

197021 (Human); 235435 (Mouse); 315750 (Rat)

Gene Symbol

LCTL

UniProt

Q6UWM7 (Human)

Additional Lactase-like Protein/LCTL Products

Product Documents for Human Lactase-like Protein/LCTL Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Lactase-like Protein/LCTL Antibody

For research use only

Citations
Reviews

Human Lactase-like Protein/LCTL Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5984

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Lactase-like Protein/LCTL Antibodies »

Product Specifications

Immunogen

Specificity