Human Kallikrein 11 Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB15951AFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 371604
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11
Specificity
Detects human Kallikrein 11 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications
Application
Recommended Usage
Neutralization
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Kallikrein 11
As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human (rh) KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), dichloroisocoumarin, and aprotinin. rhKLK11 produced by R&D Systems corresponds to isoform 1.
References
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225.
- Yousef, G.M. et al. (2000) Genomics 63:88.
- Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205.
- Gan, L. et al. (2000) Gene 257:119.
- Diamandis, E.P. et al. (2002) Cancer Res. 62:295.
- Nakamura, T. et al. (2003) Urology 61:1042.
- Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.
Alternate Names
KLK11
Entrez Gene IDs
11012 (Human)
Gene Symbol
KLK11
UniProt
Additional Kallikrein 11 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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