Detects human ISLR-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human ISLR-2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human ISLR-2 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human ISLR-2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ISLR-2

ISLR-2 (Ig-superfamily containing LRR protein 2; also KIAA1465) is a 100‑110 kDa member of the LRR family of proteins. It is expressed on developing motor, sensory and interneurons. Mature human ISLR-2 is 727 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 571 aa extracellular domain (ECD) and a 135 aa cytoplasmic region. The ECD shows multiple LRRs (aa 50‑227) and one Ig-like domain (aa 233‑371). One alternate splice form may exist that shows a deletion of aa 160‑570. Over aa 20‑590, human ISLR-2 is 89% aa identical to both canine and mouse ISLR-2.

Long Name

Immunoglobulin Superfamily Containing Leucine-rich Repeat 2

Alternate Names

ISLR2

Entrez Gene IDs

57611 (Human); 320563 (Mouse)

Gene Symbol

ISLR2

UniProt

Q6UXK2

Additional ISLR-2 Products

Product Documents for Human ISLR-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ISLR-2 Antibody

For research use only

Citations
Reviews

Human ISLR-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4526

Key Product Details

Species Reactivity

Validated:

Cited:

Applications