Human Integrin alpha5/CD49e Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1864
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Adhesion Blockade, Flow Cytometry, Simple Western, CyTOF-ready
Cited:
Western Blot, Neutralization, Immunocytochemistry, Co-Immunoprecipitation, ELISA Capture
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin alpha5/CD49e
Phe42-Tyr995
Accession # P08648
Phe42-Tyr995
Accession # P08648
Specificity
Detects human Integrin alpha5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse (rm) Integrin alpha5 and less than 5% cross-reactivity with recombinant human Integrin alphaM, rmIntegrin alpha2, rmIntegrin alpha4, and rmIntegrin alphaE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Integrin alpha5/CD49e Antibody
Detection of Human Integrin a5/CD49e by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a5/CD49e at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Integrin alpha5/CD49e by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (U87 MG) (NBP2-49844), Exosome Standards (PC-3) (NBP2-49856) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin alpha5/CD49e at approximately 165 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Integrin alpha5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Human Integrin alpha5/CD49e by Simple WesternTM.
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha5/CD49e at approximately 165 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Integrin alpha5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Integrin alpha5/CD49e Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of K562 human chronic myelogenous leukemia cells (5 x 104 cells/well) to immobilized bovine Fibronectin (Catalog # 1030-FN, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Simple Western
20 µg/mL
Sample: Exosome Standard (U87 MG) (Catalog # NBP2-49844), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Sample: Exosome Standard (U87 MG) (Catalog # NBP2-49844), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Reviewed Applications
Read 2 reviews rated 4.5 using AF1864 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 5/CD49e
Alternate Names
CD49e, ITGA5, VLA-5 alpha
Gene Symbol
ITGA5
UniProt
Additional Integrin alpha 5/CD49e Products
Product Documents for Human Integrin alpha5/CD49e Antibody
Product Specific Notices for Human Integrin alpha5/CD49e Antibody
For research use only
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