Detects human Integrin alpha5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Integrin alpha5, recombinant human (rh) Integrin alpha1, 2, 3, 4, 6, 7x2, 8, 9, 10, 11, M, X, D, or rhIntegrin beta1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Integrin alpha5/CD49e Antibody

Detection of Human Integrin alpha5/CD49e by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin a5/CD49e Monoclonal Antibody (Catalog # MAB18642) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a5/CD49e at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Integrin alpha5/CD49e Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

Integrin alpha5, also known as CD49e and vitronectin receptor subunit alpha, is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of Integrin alpha5, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) consensus repeats that fold into a beta-propeller domain. Furin cleavage of the Integrin alpha5 ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit Integrin alpha5 chain. Integrin alpha5-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2, and TSP. The ECD of human Integrin alpha5 shares 92% aa sequence identity with mouse Integrin alpha5 ECD.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

P08648

Additional Integrin alpha 5/CD49e Products

Product Documents for Human Integrin alpha5/CD49e Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Integrin alpha5/CD49e Antibody

For research use only

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Human Integrin alpha5/CD49e Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB18642

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity