IMPA1/IMP1 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Mouse Anti-Human IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB9890) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

IMPA1/IMP1 in Human Brain.

IMPA1/IMP1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB9890) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human IMPA1/IMP1 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HCT-116 human colorectal carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Reviewed Applications

Read 1 review rated 5 using MAB9890 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IMPA1/IMP1

IMPA-1/inositol(myo)-1(or 4)-monophosphatase 1 is a ubiquitous enzyme with broad specificity, which is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. IMPA-1 dephosphorylates myo-inositol monophosphatate to modulate intracellular signal transduction. IMPA-1 forms homodimers, and is abundantly expressed in brain. The magnesium-dependent phosphatase activity is inhibited by lithium, thereby depressing myo-inositol production, which may explain the anti-depressive and anti-manic effects of lithium therapy in bipolar disorder.

Long Name

Inositol Monophosphatase 1

Alternate Names

IMP, IMP 1, IMP1, IMPA, IMPase 1, MRT59, Myo-Inositol Monophosphatase 1

Entrez Gene IDs

3612 (Human); 55980 (Mouse); 83523 (Rat)

Gene Symbol

IMPA1

UniProt

P29218

Additional IMPA1/IMP1 Products

Product Documents for Human IMPA1/IMP1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IMPA1/IMP1 Antibody

For research use only

Citations
Reviews

Human IMPA1/IMP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9890

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IMPA1/IMP1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IMPA1/IMP1 Antibody

IMPA1/IMP1 in HCT‑116 Human Cell Line.