Human IL-6R alpha Antibody

Name: Human IL-6R alpha Antibody
Brand: R&D Systems
SKU: MAB2271
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB2271

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL-6R alpha Arg387-Arg468 Accession # P08887
Applications:	Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 556704
Specificity:	Detects human IL-6R alpha in direct ELISAs.

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Applications for Human IL-6R alpha Antibody

	Recommended Concentration	Sample
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-6R alpha Antibody

	Immunocytochemistry IL-6R alpha in Human PBMCs. IL-6R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB2271) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-6R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex (1‑4). Within the extracellular domain, human IL‑6 R alpha shares 52% aa sequence identity with
mouse and rat IL‑6 R alpha.

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL‑6/IL‑6 R alpha complex to membrane-bound gp130 (6, 7).

Item	Value
References	Yamasaki et al. (1988) Science 241:825. Baumann et al. (1990) J. Biol. Chem. 265:19853. Hibi et al. (1990) Cell 63:1149. Schooltink et al. (1991) Eur. J. Biochem. 277:659. Novick et al. (1989) J. Exp. Med. 170:1409. Yasukawa et al. (1990) J. Biochem. 108:673. Saito et al. (1991) J. Immunology 147:168.
Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names	CD126, IL-6 R alpha, IL-6R alpha, IL-6R-1, IL-6Ra, IL6Q, IL6R, IL6R alpha, IL6RA, IL6RQ, gp80, interleukin 6 receptor

Citations for Human IL-6R alpha Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-6R alpha Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

R Mzezewa et al. (2022), A kainic acid-induced seizure model in human pluripotent stem cell-derived cortical neurons for studying the role of IL-6 in the functional activity Stem Cell Research, 60(0):102665.
PMID: 35091307

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Customer Reviews for Human IL-6R alpha Antibody (2)

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Human IL-6R alpha Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Human PBMCs

Species: Human

Verified Customer | Posted 01/01/2022
Human IL-6 R alpha Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: THP-1 human acute monocytic leukemia cell line

Species: Human

Verified Customer | Posted 11/17/2018

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Product Documents for Human IL-6R alpha Antibody

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Human IL-6R alpha Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human IL-6R alpha Antibody

Scientific Data Examples for Human IL-6R alpha Antibody

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-6R alpha

Citations for Human IL-6R alpha Antibody (1)

Customer Reviews for Human IL-6R alpha Antibody (2)

Customer Images

Human IL-6R alpha Antibody

Human IL-6 R alpha Antibody

Product Documents for Human IL-6R alpha Antibody

Product Datasheets

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