Detects the alpha isoform of IL-32 in direct ELISAs. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) IL-32 beta and rhIL‑32 gamma is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human IL-32 alpha Antibody

Detection of Human IL‑32 alpha by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) treated (+) with 200 ng/mL Ionomycin and 10 ng/mL PMA for 72 hours. PVDF Membrane was probed with 2 µg/mL of Human IL-32a Monoclonal Antibody (Catalog # MAB30401) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-32a at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑32 alpha in Human PBMCs.

IL-32a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-32a Monoclonal Antibody (Catalog # MAB30401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human IL-32 alpha Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Western Blot

2 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC) treated with Ionomycin and PMA

Reviewed Applications

Read 1 review rated 2 using MAB30401 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-32 alpha

IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.

Long Name

Interleukin 32 alpha

Alternate Names

IL32 alpha

Entrez Gene IDs

9235 (Human)

Gene Symbol

IL32

UniProt

NP_001012651

Additional IL-32 alpha Products

Product Documents for Human IL-32 alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-32 alpha Antibody

For research use only

Citations
Reviews

Human IL-32 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB30401

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications