Human IL-3 Antibody

Name: Human IL-3 Antibody
Brand: R&D Systems
SKU: MAB603R

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB603R

Citations (1)

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL-3 Ala20-Phe152 Accession # P08700
Applications:	Block/Neutralize, ELISA Capture
Label:	Unconjugated
Source:	Recombinant Monoclonal Mouse IgG₁ Clone # 4815R
Specificity:	Detects human IL-3 in ELISAs and Western blots. In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha, rhIL-1 beta, recombinant mouse (rm) IL-1 beta, rhIL-2, rhIL-3 R alpha, rhIL-3 R beta, rmIL-3, rhIL-4, rmIL-4, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, bovine FGF acidic, bovine FGF basic, rhG-CSF, rhGM-CSF, rmGM-CSF, rhLIF, hPDGF, porcine PDGF, human TGF‑ beta 1, porcine TGF‑ beta 1, rhTGF‑ beta 1, porcine TGF‑ beta 2, porcine TGF‑ beta 1.2, rhTNF-alpha, or rhTNF-beta was observed. Does not neutralize the biological activity of rmIL‑3.

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Applications for Human IL-3 Antibody

Human IL-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human IL‑3 Biotinylated Antibody (Catalog # BAF203)

Standard: Recombinant Human IL-3 Protein (Catalog # 203-IL)

Item	Value
Neutralization	Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 1.25 ng/mL Recombinant Human IL‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-3 Antibody

	Neutralization Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 Antibody. Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Recombinant Monoclonal Antibody (Catalog # MAB603R). The ND₅₀ is typically 0.1-0.3 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

Item	Value
Long Name	Interleukin 3
Entrez Gene IDs	3562 (Human); 16187 (Mouse); 24495 (Rat)
Alternate Names	Hematopoietic growth factor, IL-3, IL-3MGC79398, IL3, MCGF, MCGFMGC79399, MULTI-CSF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell stimulating factor, P-cell-stimulating factor, interleukin 3 (colony-stimulating factor, multiple), interleukin-3, mast-cell growth factor, multilineage-colony-stimulating factor

Citations for Human IL-3 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-3 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Kankaanranta H et al. (2014), Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1. PLoS ONE, 9(2):e90298.
PMID: 24587316

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Product Documents for Human IL-3 Antibody

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Human IL-3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human IL-3 Antibody

Human IL-3 Sandwich Immunoassay

Scientific Data Examples for Human IL-3 Antibody

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-3

Citations for Human IL-3 Antibody (1)

Product Documents for Human IL-3 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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