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Human IL-3 Antibody

Catalog # MAB203 | R&D Systems, Inc. a Bio-Techne Brand
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MAB203-100
MAB203-500
MAB203-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4806

Product Summary for Human IL-3 Antibody

Immunogen

E. coli-derived recombinant human IL-3
Ala20-Phe152
Accession # Q6GS87

Specificity

Detects human IL-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse IL-3 is observed. Does not neutralize the biological activity of recombinant mouse IL-3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL-3 Antibody

Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 Antibody.

Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 Antibody.

Recombinant Human IL‑3 (Catalog # 203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB203). The ND50 is typically 0.03-0.08 µg/mL.
IL-3 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑3 in Human PBMCs.

IL-3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-3 Monoclonal Antibody (Catalog # MAB203) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human IL-3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑3 (Catalog # 203-IL)

Neutralization

Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.03-0.08 µg/mL in the presence of 1.25 ng/mL Recombinant Human IL‑3.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using MAB203 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Gene Symbol

IL3

UniProt

Additional IL-3 Products

Product Documents for Human IL-3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-3 Antibody

For research use only

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FAQs for Human IL-3 Antibody

Showing  1 - 1 of 1 FAQ Showing All
  • Q: Does Human IL-3 Antibody, Catalog # MAB203, Clone # 4806, have kappa or lambda light chain?

    A: MAB203, Clone # 4806 has kappa light chain. 

Showing  1 - 1 of 1 FAQ Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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