Human IL-20 Antibody

Name: Human IL-20 Antibody
Brand: R&D Systems
SKU: AF1102

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1102

Citations (3)

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL‑20 Leu25-Glu176 Accession # AAF36679
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL‑20 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) IL‑19 is observed, approximately 15% cross-reactivity with recombinant mouse IL‑20 is observed, and less than 1% cross-reactivity with rhIL‑10 is observed.

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Applications for Human IL-20 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑20 (Catalog # 1102-IL)

Item	Value
Neutralization	Measured by its ability to neutralize IL‑20-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑20.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-20 Antibody

	Neutralization Cell Proliferation Induced by IL‑20 and Neutralization by Human IL‑20 Antibody. Recombinant Human IL‑20 (Catalog # 1102-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 Ra and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑20 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1102). The ND₅₀ is typically 0.1-0.4 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-20

Human Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences containing a signal sequence and amphipathic helices found in helical cytokines. Human IL-20 is synthesized as a 176 amino acid (aa) precursor with a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL‑20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL‑20 share 77% aa sequence identity in their mature segments. Human IL‑20 is active on mouse cells. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL-20 R alpha and IL-20 R beta. The second is composed of IL‑22 R and IL-20 R beta. Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1-6).

Item	Value
References	Blumberg, H. et al. (2001) Cell 104:9. Liu, L. et al. (2003) Blood 102:3206. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. Dumoutier, L. (2001) J. Immunol. 167:3545. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
Long Name	Interleukin 20
Entrez Gene IDs	50604 (Human); 58181 (Mouse)
Alternate Names	Cytokine Zcyto10, IL-20, IL-20ZCYTO10four alpha helix cytokine, IL10D, IL20, MGC96907, interleukin 20, interleukin-20

Citations for Human IL-20 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-20 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

P Gough et al. (2017), IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function J. Immunol., 0(0).
PMID: 28424238
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Heinemann A et al. (2011), Induction of phenotype modifying cytokines by FERMT1 mutations. Hum. Mutat., 32(4):397-406.
PMID: 21309038
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Wolk K et al. (2009), The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur. J. Immunol., 39(12):3570-81.
PMID: 19830738

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Human IL-20 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-20 Antibody

Scientific Data Examples for Human IL-20 Antibody

Neutralization

Formulation, Preparation and Storage

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Background: IL-20

Citations for Human IL-20 Antibody (3)

Product Documents for Human IL-20 Antibody

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