Human IL-20 Antibody

Name: Human IL-20 Antibody
Brand: R&D Systems
SKU: MAB11022
Rating: 3 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB11022

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL‑20 Leu25-Glu176 Accession # AAF36679
Applications:	ELISA Capture
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 158633
Specificity:	Detects human IL-20 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse IL‑20, recombinant human (rh) IL‑20 R alpha, rhIL-20 R beta, rhIL‑22 R, or rhIL-22BP is observed.

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Applications for Human IL-20 Antibody

Human IL-20 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human IL‑20 Biotinylated Antibody (Catalog # BAF1102)

Standard: Recombinant Human IL-20 Protein (Catalog # 1102-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-20

Human Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences containing a signal sequence and amphipathic helices found in helical cytokines. Human IL-20 is synthesized as a 176 amino acid (aa) precursor with a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL‑20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 share 77% aa sequence identity in their mature segments. Human IL-20 is active on mouse cells. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL‑20 R alpha and IL‑20 R beta. The second is composed of IL‑22 R and IL‑20 R beta. Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1‑6).

Item	Value
References	Blumberg, H. et al. (2001) Cell 104:9. Liu, L. et al. (2003) Blood 102:3206. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. Dumoutier, L. (2001) J. Immunol. 167:3545. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
Long Name	Interleukin 20
Entrez Gene IDs	50604 (Human); 58181 (Mouse)
Alternate Names	Cytokine Zcyto10, IL-20, IL-20ZCYTO10four alpha helix cytokine, IL10D, IL20, MGC96907, interleukin 20, interleukin-20

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Human IL-20 Antibody

Name: Anonymous

Application: MiAr

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 12/13/2019

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma

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Human IL-20 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-20 Antibody

Human IL-20 Sandwich Immunoassay

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-20

Citations for Human IL-20 Antibody

Customer Reviews for Human IL-20 Antibody (1)

Customer Images

Human IL-20 Antibody

Product Documents for Human IL-20 Antibody

Product Datasheets

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