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Human IL‑2 R beta Antibody

Catalog # AF-224-NA | R&D Systems, Inc. a Bio-Techne Brand
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AF-224-NA
AF-224-SP

Key Product Details

Species Reactivity

Human

Applications

Neutralization, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human IL‑2 R beta Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta
Ala27-Asp239
Accession # NP_000869

Specificity

Detects human IL‑2 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity withrecombinant human (rh) IL-2 R alpha, recombinant mouse (rm) IL-2 R beta, rhIL-2 R gamma, and rhIL-15 R is observed. Is also able to block the cell surface of human IL-2 R beta mediated bioactivities induced by IL-2. For optimal neutralization of IL-2 biological activity on cells expressing the high affinity IL-2 receptors, the use of anti-IL-2 R alpha in conjunction with anti-IL-2 R beta antibodies is recommended.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL‑2 R beta Antibody

Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 R beta Antibody.

Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 R beta Antibody.

Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-224-NA). The ND50 is typically 10‑30 µg/mL.

Detection of Human IL‑2 R beta by Simple WesternTM.

Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IL‑2 R beta at approximately 98 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL‑2 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-224-NA). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human IL‑2 R beta Antibody

Application
Recommended Usage

Simple Western

25 µg/mL
Sample: HDLM‑2 human Hodgkin's lymphoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑2 R beta (Catalog # 224-2B)

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 20 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF-224-NA in the following applications:

Published Applications

Read 2 publications using AF-224-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2 R beta

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling.

IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. At present, the function of IL-2 sR beta is unclear. Recombinant human IL-2 sR beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, IL-2 sR beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

Long Name

Interleukin 2 Receptor beta

Alternate Names

CD122, IL-15 R beta, IL-2Rb, IL2R beta, IL2RB

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Gene Symbol

IL2RB

UniProt

Product Documents for Human IL‑2 R beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL‑2 R beta Antibody

For research use only

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